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Take to origin, DNA extraction, and genome sequencing

Take to origin, DNA extraction, and genome sequencing

Here because of the whole genome sequencing of 55 honey bees and also by creating a high resolution recombination map when you look at the honey bee, i unearthed that crossovers is for the GC articles, nucleotide range, and gene density. I including verified the former suggestion that family genes shown during the staff member brains have strangely high CO pricing. The investigation contain the examine that variation out of staff member choices, although not immune means, is a motorist of the highest crossing-over rate within the bees. We find no research your crossing-more price was accompanied by a leading NCO rate.

Procedures and you may content

Five colonies off honeybees (Apis mellifera ligustica Twist) was indeed built-up regarding a bee ranch when you look at the Zhenjiang, China. Each nest contained one queen, dozens of drones, and you can a huge selection of experts. Bees out of three territories was basically picked having whole genome sequencing.

Brand new DNA of each personal are extracted using phenol/chloroform/isoamyl alcohol means. Continuar leyendo